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KMID : 0381120110330050505
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Genes and Genomics
2011 Volume.33 No. 5 p.505 ~ p.505
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Identification and characterization of full-length vps29 gene in five mammalian species
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Kim Young-Hyun
Park Sang-Je
Huh Jae-Won
Kim Kyoung-Min
Lee Sang-Rae
Imakawa Kazuhiko
Chang Kyu-Tae
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Abstract
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Retromer is a heteropentameric complex associated with retrograde transport of cargo protein from the endosome to the trans-Golgi network. The mammalian retromer complex consists of three vps genes (vps26, vps35, and vps29) and two sorting nexin genes (snx1 and snx2). Previous studies had reported the protein sorting functions of retromer in the intracellular compartment. However, individual genes of retromer complex have not yet been fully characterized. In this study, we identified full-length vps29 gene in human, crab-eating macaque, mouse, rat, and dog species. Total forty-four novel transcripts of vps29, including two actively expressed major transcripts, were identified by 5′- and 3′-RACE. Comparative analysis indicated that functionally important sites of the vps29 gene were well conserved in the eukaryotic genome. However, two major transcript variants were occurred in the vertebrate genome only. From our results, we assumed that there are many different transcripts variants of vps29 gene and specifically two major transcripts could play important roles in the protein sorting mechanism.
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KEYWORD
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vps29 gene, Retromer complex, Protein sorting, Transcript variants
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